Imaging Cores - Optical

RRID:SCR_023355

Imaging Cores - Optical (About)

 

We provide access, training, and ongoing support for users of a wide range of optical (light) microscopes. From stereo and widefield microscopes to more advanced techniques like confocal, multiphoton and superresolution, the facility and the managers are available to provide technical support and consultation to advance scientific inquiry and aid in experimental design.

We have instruments and support staff in two locations:

Our location in the basement of the Marley building (main campus) is equipped with two Zeiss LSM880 34 channel Laser Scanning Confocal Microscopes. One system is an inverted laser scanning confocal microscope with seven laser lines, motorized stage, and temperature control capability. The second system is an upright laser scanning confocal/multiphoton microscope with six laser lines, motorized stage, Zeiss Fast Airyscan super-resolution attachment, and temperature control as well as a Spectra Physics MaiTai femtosecond pulsed laser for multi-photon imaging. In addition, we have a Zeiss Axiozoom 16 brightfield/fluorescent stereo microscope with a Hamamatsu Flash 4.0 16bit sCMOS camera with fluorescence filters for imaging UV (DAPI), Blue (GFP, FITC, Alexa488) and Green (RFP, Alexa 555). The facility has ancillary equipment and space to support sample preparation and sample analysis for the light microscopes.

Our location in the Life Sciences North building (UA Health Sciences) includes a fully motorized, computer controlled Leica DMI6000 inverted microscope which can be used to capture images in brightfield, DIC (mags ≥20x), polarized light, or four channels of fluorescence, using either a 16bit greyscale camera or a 24bit color camera. Life Sciences North also houses a Zeiss Axio Observer 7 with Apotome III microscope as well as a Zeiss Elyra S.1 superresolution optical microscope. The Apotome system is a multifunction widefield microscope taht can give confocal-like images of samples up to 20um in thickness. The structured illumination microscope (SIM) can be used to image fluorescence markers at twice the resolving power of confocal or deconvolution microscopes. We also provide access to and support for an image analysis workstation that features Hamamatsu's HCImage software, as well as other open-source tools.

Location

The core facility has two locations with typical work hours of:

Monday - Friday: 8:30AM - 5:00PM
(24/7 access for Trained Users)

Marley

(basement rooms 101)
1145 E 4 St.
Tucson, AZ 85721 USA

MAPS
UArizona campus map
Google maps

 
Marley building

Life Sciences North

(4th floor rooms 410B, 429)
1333 N Martin Ave
Tucson, AZ 85719 USA

MAPS
UArizona campus map
Google maps

 
Life Sciences North building

Get in touch

Image
a man wearing glasses sits in front of a computer editing an abstract image

Contact persons
Patty Jansma, M.S.
Doug Cromey, M.S.

Facility Email
opticalimaging@arizona.edu

Phone
520-621-5097 (Marley)
520-626-2824 (LSN)

Reference this core in grants and publications using:
University of Arizona Imaging Cores - Optical, RRID:SCR_023355