Imaging Cores - Optical

RRID:SCR_023355

Zeiss LSM880 Inverted Confocal Microscope

The Zeiss LSM 880 inverted confocal microscope includes a 34-channel detector for spectral information, and 7 Laser lines (405, 458, 488, 514, 561, 594, 633nm). The system includes additional software modules for performing FRAP and control for the motorized stage to allow stitching together images from multiple fields of view into one larger image (montage), as well as visiting multiple sites during a time lapse acquisition. The instrument includes an environmental chamber suitable for live cell imaging at a variety of temperatures, humidity and CO2 capabilities. The facility has wet lab space, cell culture incubators, and a biosafety hood in an adjacent room.

NOTE: BSL2 samples must be discussed with the on-site core manager BEFORE they arrive in the facility.

Objective lenses (with matching DIC prisms) include:

  • Plan-Apochromat 10x/0.45 (WD=2.0mm)
  • Plan-Apochromat 20x/0.8 (WD=0.55mm)
  • Plan-Apochromat 40x/1.3 Oil
  • Plan-Apochromat 63x/1.4 Oil
  • Plan-Apochromat 40x/0.95 dry CORR (not typically on the microscope, available upon request)
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zeiss lsm880
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