Zeiss LSM880 NLO Upright Multiphoton/Confocal Microscope with Fast Airyscan

The Zeiss LSM 880 NLO upright multiphoton/confocal microscope includes a 34-channel detector for spectral information and additional non-descanned GAsP detectors. The system includes 7 visible spectrum laser lines (405, 458, 488, 514, 561, 633 nm), plus an additional software-tunable Spectra Physics Mai Tai HP DeepSee laser (690-1040 nm). The system includes software modules for controlled region-of-interest scanning, deconvolution, physiology experiments, performing FRAP & FCS and control of the motorized stage to allow stitching together images from multiple fields of view into one larger image (montage). The software also enables visiting multiple sites during a time lapse acquisition, and the instrument includes an environmental chamber suitable for live cell imaging at a variety of temperatures. Wet lab space is available to users of the facility in an adjacent room, including cell culture incubators and a biosafety hood.

NOTE: BSL2 samples must be discussed with the on-site core manager BEFORE they arrive in the facility. Live animals - invertebrates only!

The Fast Airyscan allows for resolution improvement up to 1.9x of typical confocal resolution and the fast mode sacrifices a bit of resolution for speed (useful for live cell imaging). The scanhead includes a 34-channel detector for spectral information.