Flow Cytometry Immune Monitoring Shared Resource

RRID:SCR_023432

RII Core Facility Pilot Grants - May 1, 2024 deadline

Date of publication

Description: The RII Core Facilities Pilot Program is designed to support three key types of activity within the RII-managed core facilities. Each CFPP proposal will be evaluated on scientific merit and feasibility, and should focus on one or more of the following activity types:

  1. The development of new methods or techniques that would extend the standard operating procedures and capabilities of RII-managed core facilities and potentially impact multiple UA researchers. Successful projects will result in new methods or techniques which will be disseminated to the UA research community via the core’s website, and be incorporated into the core’s standard operating procedures and capabilities.
  2. The acquisition of preliminary data needed for extramural proposals. Successful projects will result in at least one new extramural research proposal. PIs must note the specific extramural funding opportunity announcement for which they plan to submit and their anticipated submission date along with their CFPP application. A full justification of the anticipated studies is required.
  3. The technical training of students conducting research under existing extramural funding. Successful projects will result in student researchers with the technical skills to operate equipment safely, efficiently and independently within the core facility(ies). PIs should note the student(s) to be trained, the core(s) in which they will be trained, the equipment on which they are to be trained, and the funding source under which the student’s research project is funded. Proposals to train students from traditionally marginalized groups will be given funding priority.

Due date: May 1, 2024 at 5pm
UA Cultivate link: https://arizona.infoready4.com/#competitionDetail/1915018

RII Core Facility Pilot Grants - Feb 7, 2024 deadline

Date of publication

Description: The RII Core Facilities Pilot Program is designed to support three key types of activity within the RII-managed core facilities. Each CFPP proposal will be evaluated on scientific merit and feasibility, and should focus on one or more of the following activity types:

  1. The development of new methods or techniques that would extend the standard operating procedures and capabilities of RII-managed core facilities and potentially impact multiple UA researchers. Successful projects will result in new methods or techniques which will be disseminated to the UA research community via the core’s website, and be incorporated into the core’s standard operating procedures and capabilities.
  2. The acquisition of preliminary data needed for extramural proposals. Successful projects will result in at least one new extramural research proposal. PIs must note the specific extramural funding opportunity announcement for which they plan to submit and their anticipated submission date along with their CFPP application. A full justification of the anticipated studies is required.
  3. The technical training of students conducting research under existing extramural funding. Successful projects will result in student researchers with the technical skills to operate equipment safely, efficiently and independently within the core facility(ies). PIs should note the student(s) to be trained, the core(s) in which they will be trained, the equipment on which they are to be trained, and the funding source under which the student’s research project is funded. Proposals to train students from traditionally marginalized groups will be given funding priority.

Due date: February 7, 2024 at 5pm
UA Cultivate link: https://arizona.infoready4.com/#competitionDetail/1915016 

Cytek Spectral Day

Date of publication

Join Dr. Sara Centuori and Pat Gundy for a day of educational seminars on multispectral flow cytometry and interactive learning with SpectroFlo software!

Sere the attached flier for more details. Follow this link to register. Please register by Nov 8, 2023.

Schedule:

  • 9:30am -10:00am - Coffee & bagels
  • 10:00am -10:15am - Opening remarks
  • 10:15am -10:45am - Spectral Flow Cytometry - Is It Different Than Traditional Flow? 
  • 10:45am -11:15am - Flow Cytometry Controls - Do I Really Need Them?
  • 11:30am-12:15pm - Data Analysis - OMIQ  (LUNCH PROVIDED)
  • 12:15pm-1:00pm - Great Results at the Push of a Button (aka Assay Optimization) 
  • 1:00pm-1:15pm - Closing remarks
  • 1:30pm-3:00pm - Workshop 1 - Intro to Spectral Flow Cytometry Tools and Software for Users New to High Parameter Assays 
  • 3:30pm-5:00pm - Workshop 2: Spectral Flow Cytometry Tools and Software for Users Experienced with High-Parameter Assays

Location:

  • Seminars - Cancer Center - Kiewit Auditorium
  • Workshops - Cancer Center - Conference Room 2920
Related Facility

RII Core Facility Pilot Grants - Nov 1, 2023 deadline

Date of publication

Description: The RII Core Facilities Pilot Program is designed to support three key types of activity within the RII-managed core facilities. Each CFPP proposal will be evaluated on scientific merit and feasibility, and should focus on one or more of the following activity types:

  1. The development of new methods or techniques that would extend the standard operating procedures and capabilities of RII-managed core facilities and potentially impact multiple UA researchers. Successful projects will result in new methods or techniques which will be disseminated to the UA research community via the core’s website, and be incorporated into the core’s standard operating procedures and capabilities.
  2. The acquisition of preliminary data needed for extramural proposals. Successful projects will result in at least one new extramural research proposal. PIs must note the specific extramural funding opportunity announcement for which they plan to submit and their anticipated submission date along with their CFPP application. A full justification of the anticipated studies is required.
  3. The technical training of students conducting research under existing extramural funding. Successful projects will result in student researchers with the technical skills to operate equipment safely, efficiently and independently within the core facility(ies). PIs should note the student(s) to be trained, the core(s) in which they will be trained, the equipment on which they are to be trained, and the funding source under which the student’s research project is funded. Proposals to train students from traditionally marginalized groups will be given funding priority.

Due date: November 1, 2023 at 5pm
UA Cultivate link: https://arizona.infoready4.com/#competitionDetail/1912027

Core Facility Pilot Program (August 3, 2023 deadline)

Date of publication

The RII Core Facilities Pilot Program is designed to support three key types of activity within the RII-managed core facilities. Each CFPP proposal will be evaluated on scientific merit and feasibility, and should focus on one or more of the following activity types:

  1. The development of new methods or techniques that would extend the standard operating procedures and capabilities of RII-managed core facilities and potentially impact multiple UA researchers. 
  2. The acquisition of preliminary data needed for extramural proposals. Successful projects will result in at least one new extramural research proposal. 
  3. The technical training of students conducting research under existing extramural funding. Proposals to train students from traditionally marginalized groups will be given funding priority.

Most awards will be capped at approximately $10,000, although well-justified projects may be awarded funding above this level.

List of eligible facilities:

  1. Analytical and Biological Mass Spectrometry
  2. Flow Cytometry and Human Immune Monitoring Shared Resource (BD LSR, BD, FACSARIA, BD Canto)
  3. Functional Genomics Core
  4. Imaging Cores - Electron
  5. Imaging Cores - Optical
  6. Machining and Welding Center
  7. Micro/Nano Fabrication Center
  8. Translational Bioimaging Resource
  9. University of Arizona Genetics Core
  10. BIO5 Genetically Engineered Mouse Models (GEMM) Core
  11. Arizona Genomics Institute

Proposals are due: AUGUST 2, 2023  | 5:00p 
Full Details

Monitoring the immune system to unlock new treatments

Date of publication

"As researchers work to harness the power of the immune system to fight conditions such as cancer and to understand how to control autoimmune diseases including diabetes, many labs can benefit from specialized support. The Flow Cytometry and Human Immune Monitoring Shared Resource located in the UArizona Cancer Center can help provide highly sophisticated and detailed characterization of the immune system."

"'Truly understanding the immune system is not only powerful; it is critical," said Sara Centuori, PhD, director of the shared resource and research assistant professor of medicine at the College of Medicine – Tucson. 'Immune profiling is at the forefront of understanding why diseases affect who they affect, the way they behave, and why some therapeutics work for some people but don’t for others.'”

The Flow Cytometry portion of this Shared Resource is jointly supported by the UACC and the RII Core Facilities. For more information on the FC&HIMSR on this website, follow this link.

For the full UAHS news article, follow this link.

Related Facility

RII Core Facilities Pilot Program - May 3, 2023 Deadline

Date of publication

The RII Core Facilities Pilot Program is designed to support three key types of activity within the RII-managed core facilities. Each CFPP proposal will be evaluated on scientific merit and feasibility, and should focus on one or more of the following activity types:

  1. The development of new methods or techniques that would extend the standard operating procedures and capabilities of RII-managed core facilities and potentially impact multiple UA researchers.
  2. The acquisition of preliminary data needed for extramural proposals. Successful projects will result in at least one new extramural research proposal.
  3. The technical training of students conducting research under existing extramural funding. Proposals to train students from traditionally marginalized groups will be given funding priority.

Most awards will be capped at approximately $10,000, although well-justified projects may be awarded funding above this level. It is required that CFPP proposals and their budgets be developed with the assistance of the relevant core facility manager(s). Proposals are due May 3, 2023 by 5pm. List of eligible facilities:

  1. Analytical and Biological Mass Spectrometry
  2. Flow Cytometry and Human Immune Monitoring Shared Resource (BD LSR, BD, FACSARIA, BD Canto)
  3. Functional Genomics Core
  4. Imaging Cores - Electron
  5. Imaging Cores - Optical
  6. Machining and Welding Center
  7. Micro/Nano Fabrication Center
  8. Translational Bioimaging Resource
  9. University of Arizona Genetics Core
  10. BIO5 Genetically Engineered Mouse Models (GEMM) Core
  11. Arizona Genomics Institute

Application and Full Details

New Technologies in Flow Cytometry workshop

Date of publication

The RII Flow Cytometry Shared Resource invites you to come learn about new technologies available in their lab. Lunch will be provided.

Seminars
10:00am-11:45am ImageStream (imaging flow cytometer), Presented by Amnis
12:00pm-2:00pm
Attune NxT (high throughput flow cytometry), Presented by ThermoFisher Scientific
Breakout workshops (space is limited)
3:00pm-4:00pm ImageStream Sample Demo
2:30pm-5:00pm Attune Panel Design Workshop

Registration: please register
Date: Tuesday, January 24, 2023
Location: The two seminars are in Keating 103. Workshop information is in the PDF flier.
Additional information: PDF flier

 

Contact
Related Facility

Rates

Fees effective: June 1, 2015

These rates are for Arizona Universities. For external pricing, please contact the Facility Manager or the ARL Business Office (520-621-4064).

FACSAria - Assisted Acquisision $101/hr
FACSAria - Sorting $123/hr
FACSCanto II - Assisted Acquisition $45/hr
FACSCanto II - Training $45/hr
FACSCanto II - Unassisted Acquisition $20/hr
Data Analysis - Assisted $52/hr
LSR - Assisted Acquisition $73/hr
LSR - Training $73/hr
LSR - Unassisted Acquisition $32/hr

Acquisition has priority over data reduction.

Cell-sorting users will be charged $123.00 for setup, and then $123.00 per hour for sorting prorated to 15 minute increments.

Consultation services and the use of support equipment are available free of charge.

External customers: Please contact the Core Facility Manager.

Service