Arizona Genetics Core


Next Generation Sequencing

The Arizona Genetics Core offers a variety of Illumina-supported Next-Gen Sequencing applications including Shotgun/de novo sequencing, RNAseq, Exome sequencing, Small RNA profiling as well as other custom NGS pipelines.

General Information

Sample Input

DNA or RNA from a variety of sample types can be submitted to our core.  We also offer DNA or RNA extraction for library prep.

Applications include:

  • Whole Genome Sequencing (Shotgun/de novo)
  • Metagenome Analysis
  • CHiPseq
  • miRNA profiling
  • Exome Sequencing
  • RNASeq (transcriptome sequencing)
  • Single Cell Sequencing
  • Other supported and custom protocols (please inquire)

Expected Results

  • Data output available from Illumina Miseq, NextSeq550 or NovaSeq Platforms
  • Trimming, demultiplexing and quality filtering is included at no additional charge


Data Delivery: 

Data is now returned via BaseSpace Sequence Hub. This platform offers storage and data analysis tools. Register for an account once your sequencing order is complete.

Inquire at for quote


Sample Preparation Details

DNA sample requirements (if not submitting tissue, cells, etc. for extraction):

  • 100ng-3µg total DNA required (shotgun/de novo and Exome sequencing)
  • DNA should contain no particulate matter
  • DNA should not be the result of whole genome amplification (or other process that can compromise accurate representation)
  • DNA must be double stranded
  • DNA should have an OD 260/280 ratio ≥ 1.8
  • DNA should not be degraded
  • DNA sample should be suspended in LowTE or water
  • 10+ µg total DNA for 10kb mate pair libraries
  • 10µg total DNA for 3kb and 5kb mate pair libraries

RNA sample requirements:

  • 100ng-3µg total DNA required
  • RNA should be resuspended in RNAase free water or RNA preservative
  • RNA should not be degraded


Complete the Request a Quote form and we will contact you to discuss your project workflow.

Quality Control of Samples: Every sample received for sequencing will go through a set of quality control checks before it can be processed. Customers may be asked for more DNA/RNA if their sample fails either of the following checkpoints:

  • Qubit to verify the quantity of starting material
  • Fragment Analyzer to check the material quality

Cluster Generation: Cluster generation requires 10nM post library construction concentration

Sequencing Chemistry: The Arizona Genetics Core uses Illumina NextSeq 2-channel SBS chemistry

Library Preparation/Construction: Arizona Genetics Core uses a variety of DNA/RNA library construction kits

*Note: Per Illumina recommendations we cannot run libraries that are more than 6 months old. Therefore, your library material will be disposed of 6 months after they are built in accordance with our SOPS.

Data Analysis: Supplementary data analysis will incur additional labor rates. Please contact Arizona Genetics Core to discuss further data analysis needs.


Contact Arizona Genetics Core for a quote including Library prep, Sequencing and data analysis.

Turnaround Time

Average turnaround time will vary depending upon application and sample input.

Average data turnaround time for most NGS projects is approx. 6-8weeks.

Arizona Genetics Core operates on a first come, first serve entry into sample processing queue.

Related Services

High Recovery DNA Extraction

RNA Extraction and analysis (Agilent Bioanalyzer 2100)

Sanger DNA sequencing for validation of NGS data

Additional Information

If there is a specific application you are interested in on the NextSeq 500 but do not see listed here, please contact us to see if we can accommodate your needs.

University Customers: We are located in Keating Building Room 106. Samples may be left in the freezer at the north entrance to our lab. Samples should be clearly labeled "NGS" along with your contact information and billing account.

External Customers: Samples should be shipped pre-frozen and preferably on dry ice (although regular ice packs work well enough for overnight shipping). Sample tubes should be sealed with parafilm.

Shipping Address:
Arizona Genetics Core
ATTN: Illumina NGS
1657 E. Helen St.
Keating Building, Rm 106
Tucson, AZ 85721