During the period between mid-August to mid-September all four of the major optical microscope vendors will be doing on-site demonstrations of very well equipped research-grade microscopes.
Why?
The managers of the RII Imaging Cores – Optical are evaluating microscopes, along with our faculty oversight committee, for a planned application to the UA’s equipment enhancement fund for core facilities, due September 28, 2022. The microscope would go in our core facility, probably in Life Sciences North.
We are looking for a fully-motorized, computer-controlled widefield microscope with a color camera and a high-end fluorescence camera. The microscope would be similar to the popular Leica DMI6000 that we already have, with a few distinctives. The rationale for a second, somewhat similar, microscope is to avoid potentially overloading the schedule of existing Leica.
- Stereology software – A recent faculty hire has been promised stereology software from MBF Biosciences to be installed either on the DMI6000 or possibly on the proposed new microscope. The new software controls the microscope and cameras directly, acquiring images and performing the analyses on those images in real time. Stereology is an unbiased form of image analysis that relies on the math found in geometry and statistics. The technique is often used by brain and lung researchers, but it is not limited to those organs. For more information, see: https://www.stereology.info/stereology/
- Confocal-like images – We will be evaluating three microscopes with deconvolution software and one with a hardware structured illumination approach to obtaining crisp fluorescence images. Both approaches are robust and have been used in publications for many years. One of our goals is to provide a way for UAHS labs with thin samples (e.g., paraffin sectioned tissue or cultured cells) to obtain confocal-like fluorescence images without needing to travel to the very popular confocal microscope found in the Marley facility.
For the latest information on the schedule and the vendors that are coming, please see the Microscopy Month page on the Microscopy Alliance website.