Samples for the FACScanto II and the LSR II must be provided in 12x75mm polystyrene BD brand Falcon tubes. The only tubes compatible with these analyzers are:
- Cat. # 35-2052 - sterile, no cap, 125/bag
- Cat. # 35-2054 - sterile, with cap, 125/bag
- Cat. # 35-2058 - sterile, with cap, 25/bag
- Cat. # 35-2008 - nonsterile, no cap, 1000 bulk
- Cat. # 35-2235 - sterile, with cell strainer, 25/bag
Samples for the FACSAria may be provided in 12x75mm polystyrene tubes or 15ml conical tubes.
The recommended sample concentration for the FACScanto II and the LSR II is 1x106 cells per ml in PBS (no Mg++, Ca++ or serum) or fixative.
1-3% paraformaldehyde is the recommended fixative for most cell types.
The recommended sample concentration for the FACSAria is 3x106-5x106 cells per ml in PBS (no Mg++, Ca++ or serum) or fixative.
Pathogenic samples or live samples derived from human sources must be approved by the Facility Manager. When bringing samples for multi-parameter analysis, single positive controls must be provided for each color user.